Tesamorelin + CJC-1295 + Ipamorelin + BPC-157 - APEX

1 Identity

Fixed-ratio four-component article: an N-acylated 44-residue peptide amide, a 30-residue peptide-maleimide conjugate, a five-residue pentapeptide amide with two non-proteinogenic residues, and a 15-residue all-L peptide free acid, co-lyophilized. Two of the four are GHRH(1-29)-family molecules sharing the same 29-residue scaffold, which is the fact that governs the analytics. Each component's identity lives on its own record. The second component's name is itself ambiguous in this market — 'CJC-1295' unqualified resolves either to the DAC-bearing conjugate at 3,647.250 Da or, as trade usage, to Modified GRF (1-29) at 3,367.954 Da — so this record is written for the DAC-bearing conjugate and the specification names it in full.

Contains Tesamorelin + CJC-1295 + Ipamorelin + BPC-157

Sequence

Per component; full sequences live on the component records and are not restated. Blend-relevant facts only: tesamorelin is the trans-3-hexenoyl-capped 44-mer amide, Met27 oxidation-labile; CJC-1295 (DAC:GRF) is the tetrasubstituted GHRH(1-29) scaffold extended by Lys30 carrying a 3-maleimidopropionyl group on the epsilon-amine, D-Ala2 its sole D-center; ipamorelin is Aib-His-D-2-Nal-D-Phe-Lys-NH2; BPC-157 is H-GEPPPGKPADDAGLV-OH, no cysteine and no aromatic residue. The first two share 27 of the first 29 positions, differing at position 8 (Asn against Gln), 15 (Gly against Ala), 27 (Met against Leu) and in what is attached at each terminus — which is the fact that governs the analytics.

Molecular formula

Per component; a mixture has no combined formula and none is written. Tesamorelin C221H366N72O67S free base, supplied as the acetate at roughly seven acetate equivalents; CJC-1295 (DAC:GRF) C165H269N47O46; ipamorelin C38H49N9O5; BPC-157 C62H98N16O22. Counterion is a measured finding per component and is a first-order quantity on the 44-mer.

Average mass

Average mass per component: tesamorelin 5,135.856 Da as the free base (about 5,556.2 Da as the acetate); CJC-1295 (DAC:GRF) 3,647.250 Da; ipamorelin 711.868 Da; BPC-157 1,419.556 Da. AT A nominal 10 mg each, 40 mg total, the 1:1:1:1 mass ratio is a molar ratio of 1.00 : 1.41 : 7.21 : 3.62 — 1.94710, 2.74179, 14.04755 and 7.04446 micromol. The molar figure is stated because trade documents describe such equal-mass fills as balanced. Read as no-DAC the second component would be 3,367.954 Da and the same fill would give 1.00 : 1.52 : 7.21 : 3.62, which is why the specification names the conjugate in full rather than by trade title.

Monoisotopic mass

Per component. Tesamorelin 5,132.71664 neutral, used with a deconvoluted charge envelope; CJC-1295 3,645.01548, [M+3H]3+ 1,216.0124; ipamorelin 711.3857, [M+H]+ 712.3930; BPC-157 1,418.7042, [M+H]+ 1,419.7115. The separation that SAVES this article is mass, not composition: the two GHRH-family components are 1,487.70 Da apart monoisotopic, which no instrument can miss, and that gap is the only well-conditioned handle on the pair. Putting two GHRH(1-29)-family molecules in one vial makes amino acid analysis structurally incapable of separating them — CJC-1295 has no unique residue at all, and tesamorelin's only unique residue is the one that amino acid analysis handles worst — so any per-component amino acid analysis figure for either of them that is not derived through the printed glycine-and-tyrosine difference chain has been assumed rather than measured. Mass rescues the pair; composition does not. Also required: des-acyl tesamorelin at -96.058 Da and the maleamic-acid degradant at +18.015 Da. No single blend 'molecular weight' is printed.

Salt / variant note

(1) the DAC fork on the second component: 3,647.250 against 'CJC-1295 no DAC' at 3,367.954, one name and two molecules 279.296 Da apart. (2) des-acyl tesamorelin at -96.058 Da and the isobaric acyl isomers cis-3-hexenoyl and 2-hexenoyl. (3) the maleamic-acid degradant at +18.015 Da. (4) sermorelin at 3,357.933 and Modified GRF (1-29) at 3,367.954, both routinely substituted into this product category. (5) BPC-157 salt forms — the acetate and the arginate are separate records with different gross weights. (6) Methionine sulfoxide at +15.995 Da on tesamorelin. (7) Counterion per component; ratio not standardized.

2 Class & testing panel

Form

Fixed-ratio blend

Testing panel

P2, P3 and P1 — panel definitionas a union, per component. P2 governs tesamorelin with the acyl-specific tests: intact trans-3-hexenoyl content against the des-acyl species and confirmation of double-bond position and geometry. P3 is engaged twice, D-Ala2 in CJC-1295 and Aib1/D-2-Nal3/D-Phe4 in ipamorelin, so chiral amino acid analysis runs against each component's own expected D-content, never pooled. P1 governs BPC-157. The conjugate adds two tests no panel letter carries: intact maleimide content against the maleamic-acid degradant, and a free-thiol determination

3 Primary sources & evidence

The index reports the design and provenance of the literature, not a conclusion about effect.

Literature exists for each component and is graded on that component's record. The tesamorelin file rests on registrational trials, by identifier: Falutz et al., N Engl J Med 2007;357:2359-2370, PMID 18057338, DOI 10.1056/NEJMoa072375; and Falutz et al., J Clin Endocrinol Metab 2010;95:4291-4304, PMID 20554713, DOI 10.1210/jc.2010-0490. No published study of this fixed combination at any ratio was located. None of the component literature is about this mixture.

4 Storage & specification

Storage

Co-lyophilized solid, held at -20 degrees C plus or minus 5 degrees C, desiccated, light-protected, in Type I amber glass with nitrogen headspace. Non-sterile, no sterility claim. Two written reasons rather than housekeeping: the maleimide ring hydrolyzes in the presence of water, so Karl Fischer is a release attribute; and Met27 on the 44-mer is the oxidation-sensitive point in both the solid and the reconstituted state. Any thiol-containing diluent is excluded on the label.

Shelf life

24 months from the date of manufacture, stored at -20 degrees C, desiccated and light-protected. The retest-limiting attribute is the maleimide rather than any peptide backbone: hydrolysis to the maleamic acid at +18.015 Da is the fastest chemistry in the article. Des-acyl tesamorelin at -96.058 Da and methionine sulfoxide at +15.995 Da follow.