NAD+ + MOTS-c + 5-Amino-1MQ - MitoPrime-type

1 Identity

Fixed-ratio three-component article, and only one of the three is a peptide: a 16-residue synthetic peptide free acid, a pyridine dinucleotide coenzyme, and a quaternary N-methylquinolinium salt. Two of the three carry no peptide bond anywhere. Each component's full identity lives on its own record and is cross-referenced from here rather than restated. and "NAD+ / MOTS-c / 5-Amino-1MQ stack". Two of the three names do not fix A substance: "NAD+" is sold as free acid, monosodium and disodium salts and as several hydrates, and "5-amino-1MQ" is a permanent cation always shipped as a salt whose counter-ion no seller found declares.

Contains NAD + MOTS-c + 5-Amino-1MQ

Sequence

Per component, cross-referenced. MOTS-c H-Met-Arg-Trp-Gln-Glu-Met-Gly-Tyr-Ile-Phe-Tyr-Pro-Arg-Lys-Leu-Arg-OH, MRWQEMGYIFYPRKLR, 16 residues, free acid, all-L. NAD+ has no sequence and no peptide bond: nicotinamide riboside joined to adenosine through a pyrophosphate bridge, a zwitterion with eight stereocenters and a beta-anomeric configuration whose alpha anomer is exactly isobaric. 5-Amino-1MQ has no sequence and no stereocenter at all.

Molecular formula

Per component; a mixture has no combined formula and none is written. MOTS-c C101H152N28O22S2 (free acid, salt-free). NAD+ C21H27N7O14P2 (free acid, zwitterionic inner salt); disodium salt C21H25N7Na2O14P2; hydrates declared per lot and part of the label-claim arithmetic. 5-Amino-1MQ C10H11N2+ as the cation, C10H11IN2 as the iodide - and a formula cannot be stated for a lot of it until the supplier declares the counter-ion.

Average mass

Per component, on IUPAC 2021 abridged conventional atomic weights: MOTS-c 2174.621 Da; NAD+ 663.430 Da free acid, 707.393 disodium; 5-amino-1MQ 159.212 Da as the cation and 286.117 Da as the iodide. AT A stated nominal 100 mg NAD+ plus 10 mg MOTS-c plus 50 mg 5-amino-1MQ, 160 mg total, the molar figures are 150.7318, 4.5985 and 314.0467 micromol on a cation basis - a molar ratio of 32.78 : 1.00 : 68.29. On an iodide basis the third figure falls to 174.7537 micromol, because iodide is 44.35 percent of that article by weight and 50 mg of the salt contains 27.82 mg of cation. That is a 44 percent swing in one component's molar content decided by a word the label may not carry, and it is the largest measurement error available on this article. No single blend "molecular weight" is printed.

Monoisotopic mass

Per component. MOTS-c 2173.10774 Da neutral, [M+2H]2+ 1087.56115. NAD+ 663.1091 neutral, [M+H]+ 664.1164, with NADH exactly +2.0157 and NADP+ exactly +79.9663 above it. 5-Amino-1MQ is a permanent cation observed as [M]+ at 159.0917, not as [M+H]+. Three molecules spanning 159 to 2175 Da in one vial cannot be seen in one injection on one method, and the certificate states three methods or explains how one covers a 13.7-fold mass span.

Salt / variant note

(1) ratio: none standard; the mass spread between components is the widest of any blend in this catalog. (2) NAD+ salt and hydrate: free acid 663.430, monosodium 685.411, disodium 707.393, hydrate water declared per lot; the alpha anomer is a real, separately sold and exactly isobaric impurity. (3) 5-amino-1MQ counter-ion: iodide 286.117, chloride 194.66, bromide 239.12 - a lot weighed as iodide but specified against the cation is 44 percent short of the stated substance. (4) MOTS-c: free acid against acetate 2234.67 and trifluoroacetate 2288.64; the K14Q polymorph sits 0.036 Da from the parent.

2 Class & testing panel

Form

Fixed-ratio blend

Testing panel

P1 — panel definitionfor MOTS-c and P6 for both NAD+ and 5-amino-1MQ, run per component and never once for the vial. The consequence is that net peptide content is A measurand for one component only: NAD+ and 5-amino-1MQ are assayed as w/w content against a declared salt and hydrate state, and any certificate printing net peptide content, amino acid analysis or an endotoxin figure for either of them has run a peptide template against a small molecule

3 Primary sources & evidence

The index reports the design and provenance of the literature, not a conclusion about effect.

Published literature exists for each component, is graded on that component's record and is cross-referenced rather than restated. None of it is literature about this fixed mixture, and no published specification or consensus presentation for one has been identified.

4 Storage & specification

Storage

Co-lyophilized solid in Type I amber glass with a PTFE-lined closure, nitrogen headspace and in-pack desiccant. Store at -20 degrees C plus or minus 5 degrees C, desiccated and light-protected - a non-sterile laboratory chemical carrying no sterility claim and no injection format. The labeled condition is the most restrictive of the three components rather than an average of them. The inert headspace is set by the MOTS-c oxidation inventory, and the label states that reason. NAD+ is hygroscopic; vials are equilibrated to ambient before opening.

Shelf life

Provisional 24 months at -20 degrees C plus or minus 5 degrees C from QA release, the shorter of the component intervals and provisional pending this company's own data; the blend runs its own protocol and inherits no component study. One compatibility question is answered by data rather than assumption: a redox-active dinucleotide is co-lyophilized with the most oxidation-labile peptide in this catalog. Stability-indicating attributes: per-component content, the measured ratio, MOTS-c oxidation products, NADH and alpha-anomer content, water, counterion and any new peak above 0.10 percent. Pulls at 0, 3, 6, 12, 18, 24 and 36 months.