BPC-157 + GHK-Cu + TB-500 + Thymosin Alpha-1 - GLOW Plus

1 Identity

Fixed-ratio four-component article: one copper(II) complex of a tripeptide co-lyophilized with three peptide free acids — two unmodified all-L chains and one N-alpha-acetylated all-L chain. No acylation, no disulfide, no non-proteinogenic residue anywhere in the vial. Each component's full identity lives on its own record in the catalog and is cross-referenced rather than restated. The title is the three-component GLOW article plus a fourth peptide; GLOW has its own record, and this is a different article with a different analytical problem rather than a variant of it. Two names in the title fix no molecule: "TB-500" is sold as the 43-residue acetylated chain, as the Ac-LKKTETQ heptapeptide and occasionally as Ac-SDKP, spanning 487.510 to 4,963.506 Da.

Contains BPC-157 + GHK-Cu + TB-500 + Thymosin Alpha-1

Sequence

Per component, cross-referenced. GHK-Cu: Cu(II) complex of H-Gly-His-Lys-OH. BPC-157: H-GEPPPGKPADDAGLV-OH, 15 residues. Thymosin beta-4: Ac-SDKPDMAEIEKFDKSKLKKTETQEKNPLPSKETIEQEKQAGES-OH, 43 residues. Thymosin alpha-1: Ac-SDAAVDTSSEITTKDLKEKKEVVEEAEN-OH, 28 residues. Two N-terminal acetyls and two free alpha-amines share one vial, so any Edman or free-amine check reads two of four components and reports a deficit that is not a defect.

Molecular formula

Per component; a mixture has no combined formula and none is written. GHK-Cu C14H22CuN6O4 (mono-acetate C16H26CuN6O6; free ligand C14H24N6O4). BPC-157 C62H98N16O22. Thymosin beta-4 C212H350N56O78S. Thymosin alpha-1 C129H215N33O55 (des-acetyl species C127H213N33O54). One sulfur atom exists in the whole article, on thymosin beta-4's single methionine; it is both an identity handle and the stability-limiting site. Counterion is determined per component.

Average mass

Per component, on IUPAC 2021 abridged conventional atomic weights: GHK-Cu 401.914 (mono-acetate 461.966; free ligand 340.384); BPC-157 1,419.556; thymosin beta-4 4,963.506; thymosin alpha-1 3,108.315. Worked example at a stated nominal 50 + 10 + 10 + 10 mg fill, 80 mg total: 124.405, 7.044, 2.015 and 3.217 micromol — a 61.75 : 3.50 : 1.60 : 1.00 molar ratio out of a 5 : 1 : 1 : 1 mass ratio, the widest molar spread of any article in this family. Copper is 15.81 percent of GHK-Cu by mass, so 7.905 mg of that fill is copper metal, 9.9 percent of gross. That fill is a stated nominal for arithmetic, not a market standard. No single blend molecular weight is printed; a mixture does not have one.

Monoisotopic mass

Per component, neutral: GHK-Cu 401.0999 (63Cu isotopologue); BPC-157 1,418.7042; thymosin beta-4 4,960.4863; thymosin alpha-1 3,106.5041. Only the copper component has a diagnostic envelope of its own — 63Cu to 65Cu at 69.15 : 30.85, which no organic impurity reproduces — and it is reported as measured against that ratio rather than assumed. The two large chains are observed only multiply charged and each needs a deconvoluted spectrum alongside the raw charge envelope.

Salt / variant note

(1) ratio: none standard; 5:1:1:1 by mass is a stated nominal. (2) "TB-500" identity: 4,963.506 against 889.018 against 487.510, a 10.2-fold span under one label, and which of the three a lot contains is settled by measurement before the article is specified. (3) GHK-Cu form: complex 401.914, mono-acetate 461.966, free ligand 340.384 — a lot weighed as ligand and specified as complex is 15.4 percent short. (4) counterion: acetate against trifluoroacetate, per component. (5) Des-acetyl thymosin alpha-1 is that component's characteristic synthesis failure.

2 Class & testing panel

Form

Fixed-ratio blend

Testing panel

P5 — panel definitionfor GHK-Cu and P1 for each of BPC-157, thymosin beta-4 and thymosin alpha-1, run per component and never once for the vial. The P5 element is not a quarter of the work: the metal center dictates the method hierarchy for the whole article, because the paramagnetism it contributes disables one technique for every component sharing the tube. A lot offered as recombinant thymosin beta-4 moves that component to P4 in full. Marker selection is derived from this four-component composition rather than inherited from the three-component article: two of the three markers used there do not serve here, and the replacement set is stated on the certificate together with the conditioning of its two subtraction steps. Four fully characterized component records do not by themselves characterize the blend — with no standard ratio in the market, the four-way ratio and not the chemistry is what each lot has to establish by measurement

3 Primary sources & evidence

The index reports the design and provenance of the literature, not a conclusion about effect.

Published literature exists for each component, is graded on that component's record and is cross-referenced from here rather than restated. None of it is literature about this four-component mixture: no published study of this fixed combination has been identified, and no consensus specification for it exists. A blend lot is therefore accepted on per-component evidence read alongside a measured four-way ratio, not on a citation for the combination.

4 Storage & specification

Storage

Co-lyophilized solid in Type I amber glass with a PTFE-lined closure, nitrogen headspace re-blanketed after subdivision, in-pack desiccant, held at -20 degrees C plus or minus 5 degrees C and protected from light. The labeled condition is the most restrictive of the four components, not an average of them. A non-sterile laboratory chemical: no sterility claim, no injection format. The inert headspace is chemistry rather than caution — a redox-active Cu(II) center and the article's single oxidation-labile methionine occupy the same cake.

Shelf life

Provisional at 24 months at -20 degrees C plus or minus 5 degrees C from QA release, taken as the shorter of the component intervals and provisional pending this company's own data; the blend runs its own protocol and inherits no component study. The compatibility question the study must answer rather than assume: whether co-lyophilization with a redox-active copper center moves the methionine sulfoxide profile of the thymosin beta-4 component. Stability-indicating attributes are per-component content, the four-way ratio, methionine sulfoxide, des-acetyl thymosin alpha-1, copper by ICP-MS, water and counterion, with pulls at 0, 3, 6, 12, 18, 24 and 36 months.