BPC-157 + full-length Thymosin Beta-4 - Wolverine, full-chain specification

1 Identity

Contains BPC-157 + full-length Thymosin Beta-4

Sequence

Per component; the full identity of each lives on its own record and is cross-referenced rather than restated. Component A, BPC-157: GEPPPGKPADDAGLV, 15 residues, all L, free acid, no cysteine, no aromatic residue. Component B, full-length thymosin beta-4: Ac-SDKPDMAEIEKFDKSKLKKTETQEKNPLPSKETIEQEKQAGES-OH, 43 residues, all L, N-acetylated, free acid, Met6 the single oxidation-labile residue, Phe12 the only aromatic residue in either chain.

Molecular formula

Per component, never as a sum. BPC-157 C62H98N16O22 (free acid, free base). Full-length thymosin beta-4 C212H350N56O78S (acetylated 43-mer, free acid).

Average mass

Per component: BPC-157 1,419.556 Da; full-length thymosin beta-4 4,963.506 Da. At 10 mg + 10 mg presentation: 7.044 micromol BPC-157 against 2.015 micromol thymosin beta-4, a 3.50-to-1.00 molar ratio from a 1-to-1 mass ratio.

Monoisotopic mass

Per component: BPC-157 1,418.7042 Da neutral, [M+H]+ 1,419.7115, [M+2H]2+ 710.3594. Thymosin beta-4 4,960.48632 Da neutral, taken from a deconvoluted charge envelope rather than a single ion - [M+4H]4+ 1,241.12886, [M+5H]5+ 993.10454.

Salt / variant note

Three axes of variation, each of which changes the article. (1) the thymosin fork: full-length 43-mer, 4,963.506 Da, versus the Tb4(17-23) heptapeptide Ac-LKKTETQ, 889.018 Da. (2) ratio: 10+10 mg is the commonest observed presentation but 5+5, 10+5 and 20+10 mg circulate; there is no standard. (3) salt and counterion: acetate or trifluoroacetate, chosen and stated per component, since a bis-TFA load moves gross fill weight materially without moving peptide content.

2 Class & testing panel

Form

Fixed-ratio blend

Testing panel

P1 — panel definitionfor both components, run per component rather than once on the mixture. If either component is offered as recombinant material the whole article moves to P4 - expression host declared, host-cell protein ELISA, residual host-cell DNA, SE-HPLC for aggregates

3 Primary sources & evidence

The index reports the design and provenance of the literature, not a conclusion about effect.

Published literature exists for each component and is graded, cited and identified on that component's own record. None of it is literature about this mixture.

4 Storage & specification

Storage

The labeled condition is the most restrictive of the two component conditions, not an average: co-lyophilized solid in the sealed original vial, -20 degrees C plus or minus 5 degrees C, desiccated with in-pack desiccant, protected from light, headspace displaced with nitrogen and re-blanketed after any subdivision. The nitrogen blanket is driven by Met6 in the 43-mer and is not optional because the other component is oxidation-stable. Sealed vials are equilibrated to room temperature before opening. Both components are hygroscopic. 2-8 degrees C is acceptable for transit only.

Shelf life

The company interval is 24 months at -20 degrees C plus or minus 5 degrees C from the date of QA release, taken as the shorter of the two component intervals rather than as a new number, and provisional pending this company's own data. A blend requires its own stability study and cannot inherit the component studies, because component-component interaction is not predicted from single-article data; the attributes to trend are per-component content, the measured ratio, methionine sulfoxide, water content and any new peak above 0.10 percent.