Tesamorelin + MGF + Ipamorelin - 3X

1 Identity

Fixed-ratio three-component article: an N-acylated 44-residue peptide amide, a 24-residue all-L peptide free acid corresponding to the C-terminal segment of the IGF-1Ec E-domain, and a five-residue pentapeptide amide carrying two non-proteinogenic residues, co-lyophilized. No metal center, no disulfide, no conjugate. Each component's identity lives on its own record and is cross-referenced rather than restated. It names a SKU and states no ratio. Also seen as 'Tesa/MGF/Ipa'. The second component's name is itself unstable in this market: suppliers differ on the exact boundary of the traded MGF peptide, and a 25-residue cysteine-extended form circulates under the same name.

Sequence

Per component; full sequences live on the component records and are not restated. Blend-relevant facts only: tesamorelin is the trans-3-hexenoyl-capped 44-mer amide, Met27 oxidation-labile; MGF is H-YQPPSTNKNTKSQRRKGSTFEERK-OH, 24 residues, free acid, no cysteine and therefore no disulfide - the compositional fact that separates it from the 25-mer traded under the same name - and strongly basic at three arginines, four lysines and a free N-terminus; ipamorelin is Aib-His-D-2-Nal-D-Phe-Lys-NH2. The 24-mer's boundary is confirmed against the incoming supplier's own written specification before any mass tolerance is issued.

Molecular formula

Per component; a mixture has no combined formula and none is written. Tesamorelin C221H366N72O67S free base, supplied as the acetate at roughly seven acetate equivalents; MGF C121H199N41O40 free acid, with the 25-residue cysteine-extended form C124H204N42O41S named for contrast because it imports a sulfur atom the 24-mer does not have; ipamorelin C38H49N9O5. Counterion is a measured finding per component: on a peptide with seven basic sites a trifluoroacetate MGF lot typically carries 8 to 12 percent w/w counterion.

Average mass

Average mass is given per component: tesamorelin 5,135.856 Da as the free base (about 5,556.2 Da as the acetate); MGF 2,868.170 Da; ipamorelin 711.868 Da. AT A nominal 10 mg each, 30 mg total, the 1:1:1 mass ratio is a molar ratio of 1.00 : 1.79 : 7.21 - 1.94710, 3.48654 and 14.04755 micromol. Comparators on the second component: the 25-mer cysteine-extended form 2,971.31 (+103.14), des-Arg 2,711.98 (-156.19), des-Lys 2,740.00 (-128.18).

Monoisotopic mass

Per component. Tesamorelin 5,132.71664 neutral, used with a deconvoluted charge envelope rather than a single-ion match; MGF 2,866.47980 neutral, [M+3H]3+ 956.50054, [M+4H]4+ 717.62723; ipamorelin 711.3857, [M+H]+ 712.3930. Separations that govern acceptance: des-acyl tesamorelin at -96.058 Da, invisible at nominal resolution on a 5 kDa envelope; and the 25-mer cysteine form at 2,969.48898, +103.009 monoisotopic, with the deletion sequences des-Arg 2,710.37869 and des-Lys 2,738.38484 sitting below the parent across a basic stretch that synthesizes badly. No single blend 'molecular weight' is printed.

Salt / variant note

(1) the 24-mer against the 25-mer cysteine-extended form at 2,971.31, +103.14 Da, which one captured seller publishes as its own sequence under an MGF title - and which imports a sulfur atom into a vial that should contain none. (2) PEG-MGF, a different article sold in the same category. (3) deletion sequences across the basic stretch: des-Arg 2,711.98 and des-Lys 2,740.00. (4) des-acyl tesamorelin at -96.058 Da and the isobaric acyl isomers. (5) Methionine sulfoxide at +15.995 Da on tesamorelin. (6) Counterion per component, material on a seven-basic-site peptide. (7) Ratio: '3X' names a SKU and no ratio is standard.

2 Class & testing panel

Form

Fixed-ratio blend

Testing panel

P2, P1 and P3 — panel definitionas a union, per component. P2 governs tesamorelin and brings the acyl-specific tests - intact trans-3-hexenoyl content against the des-acyl species and confirmation of double-bond position and geometry, cis-3-hexenoyl and 2-hexenoyl being exactly isobaric with it. P1 governs the 24-mer. P3 governs ipamorelin, so chiral amino acid analysis runs against its own expected D-content and never as a pooled figure; Aib is achiral and is shown present by MS/MS. No component of this article contains cysteine, so cysteic acid must be absent from the hydrolysate, and its presence is direct evidence that the 25-mer cysteine-extended form was supplied under the MGF name - the substitution one captured seller's own published sequence already discloses. Tesamorelin alone carries five unique marker residues, so each component resolves cleanly here, without the difference chains that complicate the CJC-1295-containing blends

3 Primary sources & evidence

The index reports the design and provenance of the literature, not a conclusion about effect.

Literature exists for each component and is graded on that component's record. The tesamorelin file rests on registrational trials, by identifier: Falutz et al., N Engl J Med 2007;357:2359-2370, PMID 18057338, DOI 10.1056/NEJMoa072375; and Falutz et al., J Clin Endocrinol Metab 2010;95:4291-4304, PMID 20554713, DOI 10.1210/jc.2010-0490. No published study of this fixed combination at any ratio has been identified.

4 Storage & specification

Storage

Co-lyophilized solid, held at -20 degrees C plus or minus 5 degrees C, desiccated, light-protected, in Type I amber glass with nitrogen headspace. Non-sterile, no sterility claim. The reasons are chemical rather than housekeeping: the N-terminal hexenoyl amide is the hydrolysis-sensitive point in solution and Met27 the oxidation-sensitive point in the solid; the ipamorelin naphthalene ring makes the solid photosensitive; and the 24-mer, strongly basic and hygroscopic as a trifluoroacetate, suffers most from condensed moisture, so sealed vials are equilibrated to ambient before opening.

Shelf life

24 months from the date of manufacture, stored at -20 degrees C, desiccated and light-protected. The retest-limiting attributes are des-acyl tesamorelin at -96.058 Da and methionine sulfoxide at +15.995 Da rather than backbone hydrolysis; the 24-mer has no cysteine, methionine or tryptophan, so it contributes no oxidation pathway of its own and is monitored for deamidation across its asparagine and glutamine positions.