SR9009 (Stenabolic)

1 Identity

Non-peptide small molecule. Supplier catalogs classify it as a REV-ERB (NR1D1/NR1D2) ligand originating in academic medicinal chemistry; that classification is recorded here as a catalog fact about how the article is indexed, and this record makes no statement about any effect in a person. Two structural facts govern how it is handled: the 5-nitrothiophene is a nitro-heteroaromatic structural alert of the class flagged for bacterial mutagenicity under ICH M7, and there is one stereocenter at pyrrolidine C-3. Material in circulation is understood to be racemic and no supplier consulted declares an enantiomeric composition at all, which means no supplier's specification for the article as traded is complete on that point. IUPAC name ethyl 3-({[(4-chlorophenyl)methyl][(5-nitrothiophen-2-yl)methyl]amino}methyl)pyrrolidine-1-carboxylate. Not to be confused with SR9011 (CAS 1379686-29-9) or SR9011 hydrochloride (CAS 2070014-94-5), the nearest analogs from the same medicinal-chemistry series, which are stocked on the same institutional and gray shelves under names differing by one digit. Not a peptide and not related to any peptide in this catalog.

Sequence

No defined sequence, and none can be written. This is not a peptide and contains no peptide bond, no amino acid residue and no counterion in the peptide sense. The structure is fully specified by the IUPAC name above; the identity that matters and that no seller states is the configuration at pyrrolidine C-3.

Molecular formula

C20H24ClN3O4S

Average mass

437.939 Da (C20H24ClN3O4S) on IUPAC 2021 abridged conventional atomic weights. PubChem publishes 437.9 and a reagent catalog publishes 437.94 for the same formula; all three are the same number to the precision each states. The 0.82 Da gap between average and monoisotopic mass is characteristic of a molecule carrying both chlorine and sulfur and is itself a usable identity check.

Monoisotopic mass

437.11760 Da; [M+H]+ 438.12488; [M-H]- 436.11032. The single chlorine gives a diagnostic M to M+2 envelope of approximately 100 to 32; a spectrum without it is not this molecule, and the check costs one screenshot.

Salt / variant note

(1) SR9011, CAS 1379686-29-9, the nearest analog from the same series: C23H31ClN4O3S, 479.036 avg / 478.18054 mono, 41.10 Da heavier. Two independent reagent catalogs publish that CAS at MW 479 and 479.04, agreeing with the formula. It is stocked on the same institutional shelf and the same gray shelf. (2) SR9011 hydrochloride, CAS 2070014-94-5, C23H32Cl2N4O3S, 515.494 avg / 514.15722 mono — a salt form of the analog, 77.56 Da above SR9009, and the reason a bare "479" or "515" on a certificate must be read against the stated salt. (3) The des-carboethoxy hydrolysis product, the free pyrrolidine NH after loss of the ethyl carbamate, C17H20ClN3O2S, 365.876 avg / 365.09648 mono, 72.06 Da lighter — simultaneously the obvious degradant and the obvious incomplete-synthesis residue. (4) The methyl-carbamate analog, methyl in place of ethyl, C19H22ClN3O4S, 423.912 avg / 423.10195 mono, 14.03 Da lighter — a one-carbon substitution that a nominal-mass instrument reporting "438" will not flag. (5) GW501516 (cardarine), C21H18F3NO3S2, 453.494 avg / 453.06802 mono, sold from the same shelf and only 15.56 Da away on average mass, but trivially separated by three fluorines, two sulfurs and the complete absence of chlorine — the chlorine isotope test settles it in one spectrum. (6) the variant no arithmetic can catch: the (R)- and (S)-enantiomers at pyrrolidine C-3, identical formula, identical mass at every resolving power, and no supplier states which it is selling or in what ratio. That is why a chiral method is mandatory rather than optional, and why the configuration is measured on the incoming lot rather than taken from a supplier declaration. (7) Presentation variance in the gray channel is extreme and is a substitution vector in itself: the same name is sold as bulk powder, as 10 mg capsules, as a 20 mg/mL solution and as a transdermal gel, and only the powder can be assayed against a solid-state specification at all. The company's specification for this article establishes identity by 1H and 13C NMR against an authentic standard, with mass spectrometry as corroboration rather than as the identity test, and carries ICH Q3C residual-solvent and ICH Q3D elemental-impurity limits.

2 Class & testing panel

Form

Single article

Testing panel

P6 — panel definition

3 Primary sources & evidence

The index reports the design and provenance of the literature, not a conclusion about effect.

Rodent and in-vitro literature only. The foundational report is Solt et al., Nature 2012;485:62-68, on synthetic REV-ERB agonists in mice; the subsequent corpus runs to a few dozen animal and cell papers. No controlled human trial exists and ClinicalTrials.gov returns none. The most important item for a citation index page is a negative one: Dierickx et al., PNAS 2019;116:12147-12152 reported that the compound's principal cellular effects persist in Rev-erb alpha and beta double-knockout cells, which undercuts the mechanistic attribution the compound is marketed on. The page is built to lead with that finding rather than bury it, on the same editorial rule this company applies to its own failed lots. Absences stated as absences: no pharmacokinetic characterization in humans was located, no formal toxicology or genotoxicity package was located, and no ICH M7 bacterial reverse-mutation result for the nitro-heteroaromatic core was located anywhere in the public record or in any supplier dossier examined.

4 Storage & specification

Storage

Amber glass with a desiccant sachet, tightly closed inside an opaque secondary pack, stored at -20 degrees C plus or minus 5 degrees C, desiccated and protected from light, with the container equilibrated to room temperature before opening so that moisture does not condense onto cold solid. A nitro-heteroaromatic solid of this class is light-sensitive, so the amber primary container and the opaque secondary pack are part of the specification and not a precaution. Weighing is done in a dedicated area under an occupational-exposure control band assigned from the ICH M7 structural alert this molecule carries.

Shelf life

Each lot is dated from QA release and carries a printed retest date, with first-expiry-first-out enforced against that date rather than against receipt date. The interval is set from that lot's own stability data rather than from a catalog-wide default, and the attributes that limit it are the ones this structure fails on: photodegradation of the nitro-heteroaromatic core, hydrolysis of the ethyl carbamate to the des-carboethoxy pyrrolidine, water content by Karl Fischer, total related substances by the stability-indicating chromatographic method, and enantiomeric ratio by the chiral method. Material reaching its retest date is re-assayed against those attributes and is either re-dated or destroyed on the result.