Oxytocin + PT-141 + Kisspeptin-10 - CRAVE

1 Identity

Fixed-ratio three-component article: one disulfide-bridged cyclic nonapeptide amide, one lactam-bridged cyclic heptapeptide carrying a D-residue and a non-proteinogenic residue, and one linear C-terminally amidated decapeptide. Each component's full identity lives on its own record and is cross-referenced from here rather than restated. and 'Oxytocin/PT-141/Kisspeptin'.

Contains Oxytocin + PT-141 + Kisspeptin-10

Sequence

The blend title names 'kisspeptin' without a number, which on its own does not determine a molecule. This record specifies kisspeptin-10, human, C-terminally amidated; any other reading is a different article. Per component, cross-referenced. Oxytocin H-Cys-Tyr-Ile-Gln-Asn-Cys-Pro-Leu-Gly-NH2, Cys1-Cys6 disulfide. PT-141 Ac-Nle-cyclo[Asp-His-D-Phe-Arg-Trp-Lys]-OH, lactam bridge, free acid. Kisspeptin-10 H-Tyr-Asn-Trp-Asn-Ser-Phe-Gly-Leu-Arg-Phe-NH2, an RFamide whose C-terminal primary amide is obligatory - the free acid is a different article, not a lesser grade.

Molecular formula

Per component; a mixture has no combined formula and none is written. Oxytocin C43H66N12O12S2; PT-141 C50H68N14O10 (free base); kisspeptin-10 C63H83N17O14 (free base, C-terminal amide), bis-acetate C67H91N17O18. Oxytocin is the only sulfur-containing component, so the isotope envelope isolates it; the other two are separated on mass and retention time. Kisspeptin-10 has two protonatable sites and is normally a bis-salt, so its counterion is a material share of gross vial weight and is measured, not assumed.

Average mass

Per component, on IUPAC 2021 abridged conventional atomic weights: oxytocin 1007.193 Da; PT-141 1025.182 Da; kisspeptin-10 1302.462 Da. AT A nominal 10 mg plus 10 mg plus 10 mg fill, 30 mg total, that is 9.9286, 9.7544 and 7.6778 micromol - a molar ratio of 1.2932 : 1.2705 : 1.0000. Three components inside a 300 Da band means one chromatographic method can hold all three, which is unusual in this set and is the one thing that makes this article easier to certify than its neighbors. No single blend 'molecular weight' is printed.

Monoisotopic mass

Per component. Oxytocin 1006.4365 neutral, [M+H]+ 1007.4437. PT-141 1024.5243 neutral, [M+H]+ 1025.5316. Kisspeptin-10 1301.6305 neutral, [M+H]+ 1302.6378. Kisspeptin-10's des-amido free acid is 1302.6146 neutral, which sits 0.0232 Da, about 18 ppm, from the parent's protonated ion at 1302.6378. A certificate reporting '1302.6' without naming the adduct and the charge state has not separated the product from its commonest impurity. Oxytocin adds +2.0157 on disulfide reduction and a three-way +0.9841 ambiguity of its own.

Salt / variant note

The human and murine kisspeptin-10 sequences differ by one terminal residue and about 16 daltons, and both are sold under the same name; kisspeptin-54 is a different article entirely. (1) C-terminal state: kisspeptin-10's amide is definitional and the des-amido free acid is its commonest impurity. (2) oxytocin reduced form 1009.209. (3) salt per component, kisspeptin-10 normally a bis-salt with bis-trifluoroacetate at 1530.51, of which 14.9 percent of gross weight is counterion.

2 Class & testing panel

Form

Fixed-ratio blend

Testing panel

P1 — panel definitionfor oxytocin and kisspeptin-10, P3 for PT-141, run per component and never once for the vial. The P3 element is carried by one component only and must not be applied to the other two by reflex - kisspeptin-10 contains no D-residue and no non-proteinogenic residue, and the certificate's statement that no chiral method was run on it is a disclosure rather than a gap

3 Primary sources & evidence

The index reports the design and provenance of the literature, not a conclusion about effect.

Published literature exists for each component and is graded on that component's record; two of the three additionally carry approved-product regulatory documentation. None of it is literature about this fixed three-component mixture, and no published specification for one was found.

4 Storage & specification

Storage

The article is held at minus 20 degrees C plus or minus 5 degrees C, desiccated and light-protected, with a nitrogen headspace set by the oxytocin disulfide and by two tryptophan-containing components, and with reducing conditions and elevated pH excluded.

Shelf life

Each lot is dated from QA release and carries a printed retest date, with first-expiry-first-out enforced against that date. The blend inherits no component study and carries its own stability protocol, on which the interval is set, with free thiol, scrambled disulfide, des-amido kisspeptin, deamidation and tryptophan oxidation as the stability-indicating attributes.