NALT + Kisspeptin + PT-141 + Oxytocin + VIP - Formula N-69

1 Identity

Fixed-ratio five-component article and the most heterogeneous in this set: three linear or cyclic peptides of 9, 10 and 28 residues, one lactam-bridged heptapeptide carrying a D-residue and a non-proteinogenic residue, and one N-acetylated single amino acid that contains no peptide bond at all.

Contains NALT + Kisspeptin + PT-141 + Oxytocin + VIP

Sequence

Per component, cross-referenced. Oxytocin H-Cys-Tyr-Ile-Gln-Asn-Cys-Pro-Leu-Gly-NH2, Cys1-Cys6 disulfide. PT-141 Ac-Nle-cyclo[Asp-His-D-Phe-Arg-Trp-Lys]-OH, lactam bridge. Kisspeptin-10 H-Tyr-Asn-Trp-Asn-Ser-Phe-Gly-Leu-Arg-Phe-NH2, amide obligatory. VIP H-His-Ser-Asp-Ala-Val-Phe-Thr-Asp-Asn-Tyr-Thr-Arg-Leu-Arg-Lys-Gln-Met-Ala-Val-Lys-Lys-Tyr-Leu-Asn-Ser-Ile-Leu-Asn-NH2, 28 residues, C-terminal primary amide at Asn28 annotated on UniProt P01282. NALT has no sequence: N-acetyl-L-tyrosine, one residue, acetylated on the alpha-amino nitrogen.

Molecular formula

Per component; a mixture has no combined formula and none is written. Oxytocin C43H66N12O12S2; PT-141 C50H68N14O10; kisspeptin-10 C63H83N17O14; VIP C147H238N44O42S; NALT C11H13NO4. Two of the five contain sulfur - oxytocin two atoms, VIP one at Met17 - so the isotope envelope narrows but does not resolve the set. Counterion is determined per component and independently; kisspeptin-10 is normally a bis-salt and NALT is normally supplied unsalted.

Average mass

Per component, on IUPAC 2021 abridged conventional atomic weights: NALT 223.228 Da; oxytocin 1007.193 Da; PT-141 1025.182 Da; kisspeptin-10 1302.462 Da; VIP 3325.847 Da. That is A 14.9-fold mass span in one vial and no single chromatographic method holds it; the certificate states the methods or explains the coverage. At a stated nominal 20/10/10/10/10 mg, 60 mg total, the molar figures are 89.5945, 7.6778, 9.7544, 9.9286 and 3.0068 micromol - a molar ratio of 29.80 : 2.55 : 3.24 : 3.30 : 1.00, with the smallest component by mass dominating by moles thirty to one. No single blend 'molecular weight' is printed.

Monoisotopic mass

Per component. NALT 223.08446 neutral, [M+H]+ 224.09174. Oxytocin 1006.4365, [M+H]+ 1007.4437. PT-141 1024.5243, [M+H]+ 1025.5316. Kisspeptin-10 1301.6305, [M+H]+ 1302.6378, with its des-amido free acid neutral at 1302.6146 sitting 18 ppm from that protonated ion. VIP 3323.75610, observed multiply charged, with its des-amido free acid at exactly +0.98401 - 0.33 m/z at 3+ and 0.25 at 4+, so the charge state at which the limit was demonstrated is printed.

Salt / variant note

No industry-standard ratio exists for this combination, so the proportions are the ones stated on this record and on the certificate for the lot. Three of the components, kisspeptin-10, VIP and oxytocin, are amides, and each carries its des-amido free acid at plus 0.984 Da as its commonest impurity. NALT is supplied as the free acid, and free L-tyrosine is its hydrolysis product and a specified impurity.

2 Class & testing panel

Form

Fixed-ratio blend

Testing panel

P1 — panel definitionfor oxytocin, kisspeptin-10 and VIP; P3 for PT-141; P6 for NALT, run per component and never once for the vial. Net peptide content is a measurand for four of the five components only - NALT is assayed as w/w content against a declared purity, and a certificate printing net peptide content or an amino acid analysis result for it has run a peptide template against a 223 Da small molecule

3 Primary sources & evidence

The index reports the design and provenance of the literature, not a conclusion about effect.

Published literature exists for each of the four peptide components, is graded on that component's record and is cross-referenced rather than restated; two of them additionally carry approved-product regulatory documentation. None of it is literature about this five-component mixture.

4 Storage & specification

Storage

The article is held at -20 degrees C plus or minus 5 degrees C, desiccated and light-protected, with a nitrogen headspace set by the oxytocin disulfide, the VIP methionine and two tryptophan-containing components, and with reducing conditions and elevated pH excluded.

Shelf life

Each lot is dated from QA release and carries a printed retest date, with first-expiry-first-out enforced against that date. The blend inherits no component study and carries its own stability protocol, on which the interval is set, with des-amido species on three components, free thiol, scrambled disulfide, methionine sulfoxide, tryptophan oxidation and free tyrosine from NALT hydrolysis as the stability-indicating attributes.