Modified GRF (1-29) + Hexarelin - Spartan 1

1 Identity

Fixed-ratio two-component article: two synthetic C-terminally amidated peptides, co-lyophilized. Not a molecule and not a single substance; a formulation. Both components carry D-configured positions and one carries a non-proteinogenic methylated tryptophan, so the whole article is P3 and a chiral method is mandatory. which carries the trade misnomer for the 29-mer into the blend title. Market names recorded exactly as found and not adopted as identity statements. Spartan 3 is a different article and is a separate record: it substitutes GHRP-6 for hexarelin, a des-methyl congener 14.02 Da lighter.

Contains Modified GRF (1-29) + Hexarelin

Sequence

Per component, and each component is named below by residue sequence and C-terminus. Component A, Modified GRF (1-29): Y-(D-A)-DAIFTQSYRKVLAQLSARKLLQDILSR-NH2, 29 residues, the C-terminus a primary carboxamide and not a free acid, four substitutions against native human GHRH(1-29) - D-Ala2, Gln8, Ala15, Leu27. The Leu27 substitution removes the only sulfur atom in the native sequence, which is the single most useful compositional handle on the article: any sulfur in an elemental result or isotope pattern indicates sermorelin-family contamination. Component B, Hexarelin: H-His-D-2-methyl-Trp-Ala-Trp-D-Phe-Lys-NH2, six residues, two D-configured, the position-2 residue additionally non-proteinogenic and carrying a methyl substituent at position 2 of the indole ring. Two indole side chains make the solid photolabile and oxidation-sensitive. Neither component contains cysteine, methionine, sulfur or a disulfide.

Molecular formula

Per component, never as a sum. Modified GRF (1-29) C152H252N44O42 (C-terminal amide); hexarelin C47H58N12O6 (free base); hexarelin mono-acetate C49H62N12O8, mono-trifluoroacetate C49H59F3N12O8. No combined formula is written: a mixture has no molecular formula, and a certificate printing one describes something that does not exist. Salt is stated per component; net peptide content on a trifluoroacetate lot of the 29-mer typically runs 75-85 percent.

Average mass

Per component: Modified GRF (1-29) 3,367.954 Da; hexarelin 887.059 Da, mono-acetate 947.11, mono-trifluoroacetate 1,001.08. Worked example at 10 + 10 mg, 20 mg total nominal fill: 2.969 against 11.273 micromol - a 1.00-to-3.80 molar ratio out of a 1.0-to-1.0 mass ratio. Named comparators: GHRP-6 at 873.032, exactly 14.02 Da lighter than hexarelin and roughly a third of its price; CJC-1295 with DAC at 3,647.250; sermorelin at 3,357.93. One published hexarelin figure is wrong and should not be copied: an automated read of a public reference page returned 902.03 g/mol for C47H58N12O6, which is arithmetically impossible.

Monoisotopic mass

Per component. Modified GRF (1-29) 3,365.89358 Da neutral, observed multiply charged - [M+3H]3+ 1,122.97180, [M+4H]4+ 842.48067 - reported as a deconvoluted neutral with the raw envelope shown. Hexarelin 886.4602 neutral, [M+H]+ 887.4675, [M+2H]2+ 444.2374. Comparators: GHRP-6 872.4446 neutral, 14.0157 Da lighter on the monoisotopic figure; des-amido forms of either component at plus 0.98 Da. Identity is confirmed against both expected neutrals in one run, and the 14 Da separation from GHRP-6 is the single measurement that makes this article distinguishable from Spartan 3.

Salt / variant note

(1) The hexarelin neighbor, and the governing risk on this article: GHRP-6 is hexarelin's des-methyl congener, 14.02 Da lighter, stocked more widely and roughly a third of the price. Each is a named specified impurity risk in the other, and the two are never processed on the same fill line without a cleaning-verification swab reported to the lot record. (2) The position-2 epimer of hexarelin, bearing L-2-methyl-Trp, isobaric and invisible to every achiral method. (3) The name fork on the 29-mer: 'CJC-1295 no DAC' at 3,367.954 against CJC-1295 (DAC:GRF) at 3,647.250. (4) Sermorelin at 3,357.93, containing a sulfur this article does not have. (5) Ratio: no standard exists.

2 Class & testing panel

Form

Fixed-ratio blend

Testing panel

P3 — panel definitionfor both components, run per component rather than once on the mixture. Chiral amino acid analysis must report D-Ala2 on the 29-mer and D-2-methyl-Trp2 and D-Phe5 on the hexapeptide. The hexarelin record's condition travels into the blend unchanged: the two custom reference standards that panel requires - the position-2 epimer and the des-methyl congener - must be in hand and qualified before the first lot is sampled

3 Primary sources & evidence

The index reports the design and provenance of the literature, not a conclusion about effect.

Published literature exists for each component, is graded on that component's record and is cross-referenced from here rather than restated. Hexarelin was never registered as a medicine anywhere, development having been discontinued, so its file is preclinical and early-phase. The Modified GRF (1-29) file is the GHRH-analogue literature, complicated throughout by the naming problem recorded above.

4 Storage & specification

Storage

White to off-white co-lyophilized solid in a screw-cap amber borosilicate vial with a PTFE-lined closure, desiccant sachet in the secondary pack - a laboratory-chemical presentation, non-sterile, no sterility claim, no stoppered-and-crimped injection format. Store at -20 degrees C plus or minus 5 degrees C, tightly closed, desiccated, and protected from light as a specification rather than a precaution, because the hexarelin component carries two indole side chains and is photolabile. Neither component contains cysteine, methionine or a disulfide, so inert headspace is not required and its absence is a considered position rather than an omission. Reconstituted solution is aliquoted single-use at -80 degrees C.

Shelf life

Provisional 24 months at -20 degrees C plus or minus 5 degrees C, desiccated and light-protected, from QA release, both component intervals being 24 months, and the retest date printed on each certificate is the QA release date plus that interval. Stability protocol: real-time pulls at 3, 6, 9 and 12 months at the labeled condition plus 6 months accelerated at 40 degrees C and 75 percent relative humidity, with an ICH Q1B photostability challenge run once on the launch lot in the final container. Stability-indicating attributes: the sum of indole-oxidation products at plus 16 and plus 32 Da, the hexarelin position-2 epimer, the des-amido free acid of each component, iso-aspartate on the 29-mer, per-component net content, the measured ratio, water and counterion per component. Iso-aspartate and indole oxidation are expected to be limiting rather than gross purity, and a study trending only gross purity would miss both.