GHK-Cu + Tesamorelin - Selene

1 Identity

Fixed-ratio two-component article: a copper(II) coordination complex co-lyophilized with a synthetic N-acylated 44-residue peptide amide. and "copper + Egrifta peptide" as a channel description. Market names are recorded exactly as found and are not adopted as identity statements.

Contains GHK-Cu + Tesamorelin

Sequence

Per component; each component's full identity lives on its own record and is cross-referenced here rather than restated. A, GHK-Cu: ligand H-Gly-L-His-L-Lys-OH as its copper(II) complex, conventionally 1:1. B, Tesamorelin: [trans-3-hexenoyl]-YADAIFTNSYRKVLGQLSARKLLQDIMSRQQGESNQERGARARL-NH2, 44 residues, all L, with no D-residue and no non-proteinogenic residue in the backbone, Met27 the oxidation-labile residue, and the acyl cap carrying a defined double-bond position and geometry that are both part of the identity. At 44 residues the peptide component sits above the 40-residue line at which a chain is conventionally classed as a protein rather than a peptide, and that classification sets the analytical expectations for the whole article.

Molecular formula

Per component, never as a sum. GHK-Cu C14H22CuN6O4; tesamorelin C221H366N72O67S as the free base, the supplied article being the acetate written with about seven acetate equivalents. No combined formula is written: a mixture has no molecular formula, and a certificate printing one describes something that does not exist.

Average mass

Per component. GHK-Cu 401.914 Da, on copper 63.546, an isotope-weighted average rather than the mass of any single molecule. Tesamorelin 5,135.856 Da as the free base, against which the EGRIFTA WR prescribing information states 5,135.9 and a reagent catalog publishes 5,135.78 for the same formula. With about seven acetate equivalents the salt is roughly 5,556.2 Da gross, so a content figure that does not declare free base or salt is out by about 7.6 percent. Worked example at 50 mg plus 10 mg, a 60 mg total nominal fill: 124.405 against 1.947 micromol, a 63.89-to-1.00 molar ratio out of a 5.0-to-1.0 mass ratio; copper contributes 7.905 mg, 13.18 percent of gross fill.

Monoisotopic mass

Per component. GHK-Cu 401.0999 Da neutral for the 63Cu isotopologue, the 63Cu-to-65Cu envelope at 69.15 to 30.85 being part of the identity. Tesamorelin 5,132.71664 Da as the free base, to be used with a deconvoluted charge envelope rather than with a single-ion match, since at this mass the monoisotopic peak is not the base peak of the isotope envelope.

Salt / variant note

The acyl isomer question, which is the one most often left unanswered: cis-3-hexenoyl and 2-hexenoyl are exactly isobaric with the specified trans-3-hexenoyl cap, so a document that never names the isomer has not tested for it. The copper fork: 1:1 complex 401.914, mono-acetate 461.966, metal-free GHK 340.384, AHK-Cu 415.94. Salt: tesamorelin acetate at about seven equivalents against the free base, a 7.6 percent difference in declared content. Ratio: no standard presentation exists. Tesamorelin is the active moiety of an approved United States product marketed as EGRIFTA, and also as EGRIFTA SV and EGRIFTA WR, under NDA 022505 held by Theratechnologies Inc. And approved 10 November 2010; that brand family is recorded here as identity, so that the names are not read as separate molecules.

2 Class & testing panel

Form

Fixed-ratio blend

Testing panel

P5 and P2 — panel definitiontogether, as a union rather than the higher of the two. P5 governs the copper complex — copper stoichiometry by ICP-MS, the Cu(II) d-d band, the copper isotope envelope. P2 governs the acylated peptide — the trans-3-hexenoyl cap identified by position and geometry, cis-3-hexenoyl and 2-hexenoyl being exactly isobaric with it and separable only chromatographically. Each panel runs on the component it governs, per component and never once on the blended vial, and the peptide component is reported as a deconvoluted neutral with the raw charge envelope shown

3 Primary sources & evidence

The index reports the design and provenance of the literature, not a conclusion about effect.

Published literature exists for each component, is graded on that component's record and is cross-referenced from here rather than restated. The tesamorelin literature is the sponsor's development program behind NDA 022505, a single coherent body of work rather than a scattered one. The GHK-Cu literature is graded D and includes Pickart and Margolina, Cosmetics 2015, DOI 10.3390/cosmetics2030236. None of it is literature about this mixture: no published study of this fixed two-component combination was located.

4 Storage & specification

Storage

Blue to blue-violet co-lyophilized solid in a Type I amber glass vial with a PTFE-lined closure. Store at -20 degrees C plus or minus 5 degrees C, desiccated, protected from light, with the headspace displaced with nitrogen for the methionine — Met27 is the oxidation-labile residue, so the inert headspace is a specification and not a housekeeping preference. Color is recorded at each handling step as an in-process identity check, the blue-violet arising from the copper d-d absorption; drift toward pale, green or brown is investigated.

Shelf life

The dating condition is the storage condition: -20 degrees C plus or minus 5 degrees C, desiccated, protected from light, headspace nitrogen-displaced, with the interval dated from QA release. A blend of this kind runs its own stability protocol rather than inheriting either component's interval, and the interval is set on this company's own real-time and accelerated data rather than on a supplier's certificate. The retest-limiting attributes come from the peptide component: oxidation at Met27, the acyl cap held to both position and geometry, per-component net content, the measured ratio, copper stoichiometry, water and counterion.