GHK-Cu + Epitalon - Fountain of Youth, formula two

1 Identity

Fixed-ratio two-component article: a copper(II) coordination complex co-lyophilized with a synthetic linear tetrapeptide. and "GHK-Cu + Epitalon". Formula one is A different article — four components, adding BPC-157 and TB-500 — and is a separate record. A name that resolves to two compositions is not an identity statement, which is why the formula number sits in the title and why "Fountain of Youth" alone is never accepted on an order.

Sequence

Per component; each component's full identity lives on its own record and is cross-referenced here rather than restated. A, GHK-Cu: ligand H-Gly-L-His-L-Lys-OH as its copper(II) complex, conventionally 1:1, the metal integral to the article rather than an additive. B, Epitalon: H-Ala-Glu-Asp-Gly-OH (AEDG), all L, free acid, Gly4 achiral so three stereocenters. Neither component carries an aromatic residue, so this article has no 280 nm chromophore at all, and a detector set at 280 nm sees nothing of either component.

Molecular formula

Per component, never as a sum. GHK-Cu C14H22CuN6O4 for the 1:1 complex free of added counterion; mono-acetate C16H26CuN6O6. Epitalon C14H22N4O9 as the free acid. No combined formula is written: a mixture has no molecular formula, and a certificate printing one describes something that does not exist. One coincidence is named so that it is not mistaken for an error — both components share the fragment C14H22, differing only in Cu-N6O4 against N4O9, so elemental composition alone does not distinguish this pair.

Average mass

Per component. GHK-Cu 401.914 Da, computed on the conventional atomic weight of copper, 63.546, which is an isotope-weighted average and not the mass of any single molecule; mono-acetate 461.966; free GHK ligand 340.384. Epitalon 390.349 Da. The two components sit only 11.565 Da apart, closer to each other than either is to its own commonest impostor. Worked example at 50 mg plus 10 mg, a 60 mg total nominal fill: 124.405 against 25.618 micromol, a 4.86-to-1.00 molar ratio out of a 5.0-to-1.0 mass ratio; copper contributes 7.905 mg, 13.18 percent of gross fill. The certificate states which ratio it reports.

Monoisotopic mass

Per component. GHK-Cu 401.0999 Da neutral for the 63Cu isotopologue, [M+H]+ 402.1072; the 65Cu isotopologue sits 1.998 Da higher at 403.0980, and the 63Cu-to-65Cu envelope at 69.15 to 30.85 is part of the identity rather than an instrumental artifact. Free GHK ligand 340.1859. Epitalon 390.13868 neutral, [M-H]- 389.13140; negative mode is the sensible primary on a tetrapeptide carrying three carboxylates against one amine. Identity is confirmed against both expected neutrals in one run.

Salt / variant note

The copper fork, three of whose members circulate under the name "GHK-Cu": the 1:1 complex at 401.914; the mono-acetate at 461.966; metal-free GHK at 340.384, which contains no copper at all and whose powder is white rather than blue-violet; AHK-Cu at 415.94, exactly +14.03 Da away; and prezatide copper acetate at 800.33, a 2:1 bis-complex. The epitalon neighbor: Vesugen sits 0.044 Da away on average mass, and resolving that pair is a validated-method condition that a blend does not relax; Cartalax (AED, 333.297 Da) is Epitalon less one glycine. Salt: acetate or trifluoroacetate, stated per component. Ratio: no standard presentation exists for this pairing. The name resolves to two compositions in this catalog, which is why the formula number is carried in the title.

2 Class & testing panel

Form

Fixed-ratio blend

Testing panel

P5 and P1 — panel definitiontogether, as a union rather than the higher of the two. P5 governs the copper complex — copper stoichiometry by ICP-MS, the Cu(II) d-d band, the copper isotope envelope, and salt form stated as a measured finding rather than restated from a label. P1 governs the tetrapeptide. The article is released against both panels in full and per component: each panel runs on the component it governs, separately, and never once on the blended vial. This is the best-conditioned copper blend in the catalog for that per-component work, because histidine and lysine are unique to GHK-Cu while alanine, glutamate and aspartate are each unique to Epitalon, so every component has a marker residue the other does not carry. The four-component copper blends in this catalog do not have that, GHK-Cu carrying no unique proteinogenic marker in them

3 Primary sources & evidence

The index reports the design and provenance of the literature, not a conclusion about effect.

Published literature exists for each component, is graded on that component's record and is cross-referenced from here rather than restated. The GHK-Cu literature is graded D and carries identifiers including Pickart and Margolina, Cosmetics 2015, DOI 10.3390/cosmetics2030236, and Dymek et al., Pharmaceutics 2023, DOI 10.3390/pharmaceutics15102485. The Epitalon literature is the Khavinson short-peptide bioregulator body of work, predominantly Russian-language and predominantly from a single research group. None of it is literature about this mixture: no published study of this fixed two-component combination at any ratio was located.

4 Storage & specification

Storage

Blue to blue-violet co-lyophilized solid in a Type I amber glass vial with a PTFE-lined closure — a laboratory-chemical presentation, non-sterile, with no sterility claim and no stoppered-and-crimped injection format. Store at -20 degrees C plus or minus 5 degrees C, tightly closed, desiccated, protected from light. Color is a real in-process identity check on this article and is recorded at each handling step, the blue-violet arising from the copper d-d absorption near 620-640 nm; drift toward pale, green or brown is investigated. Sealed vials are equilibrated to ambient before opening. The article is held physically segregated from the metal-free GHK article, that particular cross-contamination being invisible on an RP-HPLC trace. The copper complex is incompatible with strong chelators, reducing agents and phosphate buffers, and the compatible diluent is named on the label.

Shelf life

Provisional 24 months at -20 degrees C plus or minus 5 degrees C, desiccated and protected from light, from the date of QA release — the shorter of the two component intervals, both being 24 months — and provisional pending this company's own data. The blend runs its own stability protocol and does not inherit the component studies, with a mandatory 6-month interim pull on each of the first three lots. Stability-indicating attributes: per-component net content, the measured ratio, copper stoichiometry by ICP-MS, the d-d band lambda-max, free-ligand GHK at 340.186 as the named transchelation marker — Epitalon is itself a carboxylate-rich potential ligand for Cu(II), so transchelation inside the vial is a real pathway and is watched at that mass — the Asp3 succinimide and iso-aspartate species, water, and counterion. Neither component contains cysteine, methionine, tryptophan or a disulfide, so oxidation is not a pathway here and an oxidation panel on this article would be a method copied from elsewhere.