DSIP + BPC-157 + CJC-1295 - Sleep

1 Identity

Fixed-ratio three-component article: two unmodified all-L peptide free acids co-lyophilized with one C-terminally amidated analogue chain bearing a D-amino acid at position 2 and a maleimide conjugation handle on a lysine side chain. No metal center, no disulfide, no free thiol anywhere in the vial. Each component's full identity lives on its own record and is cross-referenced from here rather than restated. One component name covers two molecules and this record specifies which: CJC-1295 is sold both as DAC:GRF, the 30-residue maleimidopropionyl conjugate at 3,647.250 Da, and as CJC-1295 no DAC, which is Modified GRF (1-29) at 3,367.954 Da. This record is written for the DAC-bearing conjugate and does not accept the bare abbreviation, because the conjugate-specific test set is meaningless on the no-DAC article.

Sequence

Stated per component. DSIP is H-WAGGDASGE-OH, nine residues, all L. BPC-157 is H-GEPPPGKPADDAGLV-OH, 15 residues. CJC-1295 (DAC:GRF) is H-Tyr-D-Ala-Asp-Ala-Ile-Phe-Thr-Gln-Ser-Tyr-Arg-Lys-Val-Leu-Ala-Gln-Leu-Ser-Ala-Arg-Lys-Leu-Leu-Gln-Asp-Ile-Leu-Ser-Arg-Lys30(N-epsilon-3-maleimidopropionyl)-NH2, 30 residues. Two free acids and one amide share the vial, so a single C-terminal check reads one component and not the article.

Molecular formula

Stated per component. A mixture has no combined formula and none is written. DSIP C35H48N10O15 as the free acid, the acetate properly written C35H48N10O15 . XC2H4O2. BPC-157 C62H98N16O22. CJC-1295 (DAC:GRF) C165H269N47O46; the no-DAC article, where that is what a label means, is C152H252N44O42. No sulfur atom exists anywhere in this vial under either CJC reading, which is an analytical asset: the isotope envelope of every component carries no 34S contribution, so an unexplained 34S signature is contamination and not chemistry.

Average mass

Per component, on IUPAC 2021 abridged conventional atomic weights: DSIP 848.824; BPC-157 1,419.556; CJC-1295 (DAC:GRF) 3,647.250, against Modified GRF (1-29) at 3,367.954 where the no-DAC article is meant. Worked example at a stated nominal 10 + 10 + 10 mg fill, 30 mg total: 11.781, 7.044 and 2.742 micromol, a 4.30 : 2.57 : 1.00 molar ratio out of a 1 : 1 : 1 mass ratio. That fill is a stated nominal for arithmetic and not a market standard. No single blend molecular weight is printed; a mixture does not have one.

Monoisotopic mass

Per component, neutral: DSIP 848.3301; BPC-157 1,418.7042; CJC-1295 (DAC:GRF) 3,645.0155; Modified GRF (1-29) 3,365.8936. The CJC component's diagnostic delta is +18.011 Da, the ring-opened maleamic acid formed by maleimide hydrolysis, and it is reported as a named impurity rather than absorbed into a purity total. The two small components are readily observed singly and doubly charged; the conjugate is observed multiply charged and requires a deconvoluted spectrum alongside the raw envelope.

Salt / variant note

(1) ratio: none standard across listings. (2) the CJC-1295 identity fork: DAC:GRF at 3,647.250 against no-DAC Modified GRF (1-29) at 3,367.954, a 279.296 Da separation and the one substitution that changes which test set applies. (3) DSIP nomenclature: carried in institutional catalogs as emideltide, CAS 62568-57-4 and 69431-45-4, UNII YN28Z5YZ73; a search on the abbreviation alone returns nothing, and that is a search artifact rather than an absence. (4) counterion: acetate against trifluoroacetate, determined per component. (5) the maleimide state: intact ring against the hydrolysis product at +18.011 Da on the CJC component, a degraded lot of this article rather than a different one.

2 Class & testing panel

Form

Fixed-ratio blend

Testing panel

P1 — panel definitionfor DSIP and BPC-157, P3 for CJC-1295, run per component and never once for the vial. The P3 element belongs to one component only: chiral amino acid analysis for D-Ala2 and conjugate-specific testing for the maleimidopropionyl group are CJC-1295 determinations, and running them once for the vial reports a chirality figure and a conjugate figure diluted by two components that have neither. Amino acid analysis has a further constraint on this article: DSIP carries no acid-stable unique residue, its tryptophan not surviving standard acid hydrolysis, so DSIP content is reached by difference against independently fixed component quantities, and that dependency is stated on the specification rather than left inside the method. Per-component net content and the three-way ratio are separate determinations

3 Primary sources & evidence

The index reports the design and provenance of the literature, not a conclusion about effect.

What follows reports study design and provenance, not a conclusion about effect. Published literature exists for each of the three components, sits on that component's own record in this catalog with its design and identifiers, and is cross-referenced here rather than restated. None of it is literature about this three-component mixture. Combination literature: none identified. No published study of this fixed combination at any ratio was identified, and no consensus specification for the combination was identified; that is a statement about the published record and not a conclusion about any component.

4 Storage & specification

Storage

The labeled condition is the most restrictive of the three components: co-lyophilized solid in Type I amber glass with a nitrogen headspace, stored at -20 degrees C plus or minus 5 degrees C, desiccated and protected from light. Non-sterile laboratory chemical, no sterility claim, no injection format. The moisture control is set by the CJC component rather than by the two peptides: maleimide hydrolysis to the maleamic acid is a water-mediated reaction, so residual moisture in the cake is a reaction substrate and not merely a specification line, and the desiccant and the close-immediately-after-weighing instruction are part of the specification.

Shelf life

Provisional 24 months at -20 degrees C plus or minus 5 degrees C, desiccated and light-protected, dated from QA release. It is the shorter of the component intervals and provisional pending this company's own data; the blend runs its own protocol and inherits no component study. The compatibility question the study answers rather than assumes: whether the free lysine epsilon-amines carried by BPC-157 and by CJC-1295 itself react with the maleimide over shelf life, which is a slower pathway than thiol addition but not a closed one. Stability-indicating attributes: per-component content, the three-way ratio, maleimide ring-opening at +18.011 Da, DSIP aspartate-linked degradation, water, counterion and any new peak above 0.10 percent. Pulls at 0, 3, 6, 12, 18, 24 and 36 months.