BPC-157 + TB4 Fragment 1-4 + GHK-Cu - Healing and Repair-type

1 Identity

Fixed-ratio three-component article: two plain linear peptide free acids and one copper(II) coordination complex of a tripeptide ligand. The metal is the fact that reorganizes the whole specification. TB4 fragment 1-4 IS Ac-SDKP and is not TB-500: three different thymosin beta-4 articles circulate under overlapping names — the full-length 43-residue chain, the 17-23 heptapeptide and this N-terminal tetrapeptide — and they differ by kilodaltons.

Sequence

Per component, cross-referenced; each component's full identity lives on its own record. BPC-157 H-Gly-Glu-Pro-Pro-Pro-Gly-Lys-Pro-Ala-Asp-Asp-Ala-Gly-Leu-Val-OH, 15 residues, free acid. Ac-SDKP Ac-Ser-Asp-Lys-Pro-OH, four residues, the acetyl on the serine alpha-amino nitrogen and localized by MS/MS b1 and b2 assignment rather than inferred from intact mass — thymosin beta-4 residues 1 to 4. GHK-Cu ligand H-Gly-L-His-L-Lys-OH, held as a 1:1 copper(II) complex through a three-nitrogen donor set.

Molecular formula

Per component; a mixture has no combined formula and none is written. BPC-157 C62H98N16O22; Ac-SDKP C20H33N5O9 as the free acid, acetate salt C22H37N5O11, trifluoroacetate C22H34F3N5O11; GHK-Cu C14H22CuN6O4 for the 1:1 complex, free GHK ligand C14H24N6O4, mono-acetate C16H26CuN6O6. The copper is part of the article and not an impurity, and its stoichiometry is a stated attribute; the free ligand at 340.38 is a different substance from the complex at 401.91.

Average mass

Per component, on IUPAC 2021 abridged conventional atomic weights: BPC-157 1419.556 Da; Ac-SDKP 487.510 Da; GHK-Cu 401.914 Da, of which copper is 15.81 percent by weight. AT A stated nominal 10 mg plus 10 mg plus 50 mg, 70 mg total, that is 7.0445, 20.5124 and 124.4047 micromol — a molar ratio of 1.0000 : 2.9119 : 17.6599, and the 50 mg of complex carries 7.91 mg of elemental copper into the vial. No single blend molecular weight is printed. Copper's conventional atomic weight of 63.546 is an isotope-weighted average and is not the mass of any single molecule.

Monoisotopic mass

Per component. BPC-157 1418.7042 neutral, [M+H]+ 1419.7115. Ac-SDKP 487.22783 neutral, [M+H]+ 488.23510. GHK-Cu 401.0999 neutral for the 63Cu isotopologue, [M+H]+ 402.1072, with the 65Cu isotopologue at 403.0980, 1.998 Da higher. The copper isotope envelope, 63Cu TO 65Cu AT 69.15 TO 30.85, is part of the identity of that component and not an instrumental artifact, and a monoisotopic figure quoted without naming the isotopologue is incomplete.

Salt / variant note

(1) ratio: none standard. (2) the thymosin beta-4 fork: the full-length 43-residue chain, the 17-23 heptapeptide at 889.018 and this 1-4 tetrapeptide at 487.510, all traded under overlapping names. (3) the GHK-Cu FORK: free GHK ligand 340.38 against the 1:1 complex 401.91 against the mono-acetate 461.97 — a 60 Da and 15.8 percent copper difference that no seller found declares. (4) BPC-157 salt: acetate, trifluoroacetate and the L-arginine salt, mass-spectrometrically indistinguishable. (5) Ac-SDKP: free acid, acetate 547.562, trifluoroacetate 601.533.

2 Class & testing panel

Form

Fixed-ratio blend

Testing panel

P1 — panel definitionfor BPC-157 and Ac-SDKP, P5 for GHK-Cu, run per component and never once for the vial. The P5 element governs the other two by contamination rather than by chemistry: free Cu(II) in the vial interferes with AQC and OPA derivatization, so a desalting or EDTA step is written into the amino acid analysis method for the whole article and is not left to the laboratory's discretion. The ICP-MS cross-check is written against the copper arithmetic above, 15.81 percent by weight and 7.91 mg of elemental copper in a 50 mg component

3 Primary sources & evidence

The index reports the design and provenance of the literature, not a conclusion about effect.

Published literature exists for each component, is graded on that component's record and is cross-referenced rather than restated. None of it is literature about this three-component mixture. No published study of this fixed combination, and no consensus specification for it, has been identified, which is a statement about the search.

4 Storage & specification

Storage

Co-lyophilized solid, the GHK-Cu component blue to blue-violet and coloring the cake, in Type I amber glass with a PTFE-lined closure, nitrogen headspace, in-pack desiccant, held at -20 degrees C plus or minus 5 degrees C, desiccated and protected from light. Non-sterile laboratory chemical. Vials are equilibrated to ambient before opening.

Shelf life

Provisional 24 months at -20 degrees C plus or minus 5 degrees C from QA release, the shortest of the three component intervals, set on this company's own data and inheriting no component study. One compatibility question is answered by data and not by assumption: a redox-active copper complex is co-lyophilized with two peptides, and whether that changes their degradation profile is measured. Stability-indicating attributes: per-component content, the measured ratio, copper-to-ligand stoichiometry, free ligand, the mass-silent aspartyl-to-isoaspartyl rearrangement in both BPC-157 and Ac-SDKP which requires a chromatographic method, water, counter-ion and any new peak above 0.10 percent. Pulls at 0, 3, 6, 12, 18, 24 and 36 months.