AOD-9604 + MOTS-c - Lean

1 Identity

Fixed-ratio two-component article: two all-L peptide free acids co-lyophilized, one carrying a single intramolecular disulfide and one carrying none. No metal center, no acyl chain, no conjugate, no non-proteinogenic residue. Each component's full identity lives on its own record in the catalog and is cross-referenced rather than restated. and "AOD + MOTS" — market titles. Neither name fixes A salt: both components circulate as acetate and as trifluoroacetate and most listings write neither. "MOTS-c" further covers five articles across the market — the native human 16-mer, the K14Q polymorph peptide, rat and mouse sequences and the (1-12) and (1-13) fragments — and a blend title inherits that ambiguity whole.

Sequence

Per component, cross-referenced. AOD-9604: H-YLRIVQCRSVEGSCGF-OH, 16 residues, one intramolecular disulfide Cys7-Cys14 in this numbering, corresponding to hGH Cys182-Cys189. MOTS-c: H-MRWQEMGYIFYPRKLR-OH, 16 residues, free acid, no cysteine. Two sixteen-residue peptides in one vial is a coincidence of length and nothing else; they are 359.5 Da apart and share no terminal chemistry problem.

Molecular formula

Per component; a mixture has no combined formula and none is written. AOD-9604 C78H123N23O23S2 (disulfide-closed free base). MOTS-c C101H152N28O22S2 (free acid); mono-acetate C103H156N28O24S2; mono-trifluoroacetate C103H153F3N28O24S2; mono-sulfoxide C101H152N28O23S2. Both components carry exactly two sulfur atoms and they are not the same kind: two cysteine thiols closed as one disulfide against two methionine thioethers. A total-sulfur result therefore separates nothing and is not accepted as a ratio method.

Average mass

Per component, on IUPAC 2021 abridged conventional atomic weights: AOD-9604 1,815.100; MOTS-c 2,174.621 (mono-acetate 2,234.67; mono-trifluoroacetate 2,288.64). Worked example at a stated nominal 10 + 10 mg fill, 20 mg total: 5.509 and 4.599 micromol, a 1.198 : 1.000 molar ratio out of a 1 : 1 mass ratio. Equal mass is not equal moles and the certificate states which basis the ratio criterion is written on. That fill is a stated nominal for arithmetic; the observed channel standard for AOD-9604 alone is a 5 mg vial. No single blend molecular weight is printed; a mixture does not have one.

Monoisotopic mass

Per component, neutral: AOD-9604 1,813.8604; MOTS-c 2,173.1077. Both are observed multiply charged and each requires a deconvoluted spectrum alongside the raw charge envelope. Two named deltas are checked rather than assumed: a reduced disulfide on AOD-9604 at +2.0157 and a single methionine sulfoxide on MOTS-c at +15.9949. A plus or minus 5 ppm identity window excludes neither, so intact mass is read alongside the peptide map rather than instead of it.

Salt / variant note

(1) ratio: none standard; the single-article channel sells AOD-9604 at 5 mg and MOTS-c at 10, 20 and 40 mg, so a 1:1 fill is a formulator's choice rather than a market convention. (2) MOTS-c identity: five articles under one name; the K14Q polymorph peptide sits 0.036 Da from the parent and is not resolved by intact mass at any practical resolution. (3) counterion: acetate 2,234.67 against trifluoroacetate 2,288.64 on MOTS-c. (4) AOD-9604 redox state: disulfide-closed against reduced, 2.0157 Da, an identity attribute rather than an impurity line.

2 Class & testing panel

Form

Fixed-ratio blend

Testing panel

P1 — panel definitionfor both components, run per component and never once for the vial, with one disulfide attribute contributed by AOD-9604 alone. A single free-thiol line for the vial is meaningless: the number belongs to AOD-9604, the other component containing no cysteine at all, and it is reported against that component. The sulfur symmetry sits on the face of this record for the matching reason — two components carrying two sulfur atoms each is the specific case in which an elemental method looks like a ratio method and is not. Tryptophan is expressly excluded as an amino acid analysis marker on this article, so that a laboratory cannot select it and report as a content shortfall what is a hydrolysis artifact

3 Primary sources & evidence

The index reports the design and provenance of the literature, not a conclusion about effect.

Published literature exists for each component, is graded on that component's record and is cross-referenced from here rather than restated. None of it is literature about this two-component mixture: no published study of this fixed combination has been identified, and no consensus specification for it exists. A blend lot is therefore accepted on per-component evidence read alongside a measured ratio, not on a citation for the combination.

4 Storage & specification

Storage

Co-lyophilized solid at -20 degrees C plus or minus 5 degrees C, desiccated, protected from light, nitrogen headspace re-blanketed after subdivision. The labeled condition is the more restrictive of the two components rather than an average of them. The inert headspace is set by MOTS-c, whose two methionines, one tryptophan and two tyrosines make it the most oxidation-labile article in the vial, and by AOD-9604's disulfide, a reducible bond sharing a cake with oxidizable residues.

Shelf life

Dating runs on the blend's own stability protocol; the article inherits no component study. The compatibility question that protocol answers rather than assumes is whether co-lyophilizing a disulfide-containing peptide with a methionine-rich peptide moves either the free-thiol or the sulfoxide trajectory. The stability-indicating attributes are per-component content, the measured ratio, AOD-9604 free thiol and disulfide scrambling, MOTS-c sulfoxide and tryptophan oxidation, water and counterion.